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Beckman贝克曼 纯化磁珠 核酸纯化试剂60ml(A63881)

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Beckman贝克曼 核酸纯化试剂60ml(A63881)纯化磁珠

 

 

 

 

Beckman贝克曼 纯化磁珠 核酸纯化试剂60ml(A63881)

 

 

 

 

 

 

 

1双链和单链DNA都能得到纯化


2、有效去除dNTP、引物、引物二聚体、盐离子和其它杂质


2、200bp以上的核酸回收率高达90%以上,核酸长度最小要求100bp


3、纯化后的产物在4℃温度下保持7天不被降解


4、适用于PCR体系纯化、酶切和连接反应体系纯化

 

 

Application: PCR purification

Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

Agencourt AMPure XP Features

  • High recovery of amplicons, greater than 100 bp
  • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
  • Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
  • Efficient recovery of double stranded and single stranded DNA templates
  • Consistent recovery throughout the kit’s 12 month shelf life
  • Faster manual and automated processing as compared to traditional post-PCR cleanup methods

Process Overview

 

1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

High Recovery

Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).

 

Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

Walk-away Automated Solution

Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NXP and FXP for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.

Ordering Information

Product Size Kit Components

Product #

Agencourt AMPure XP 5 mL Kit 139/278 preps1 Agencourt AMPure XP Reagent

A63880

Agencourt AMPure XP 60 mL Kit 1,666/3,333 preps1 Agencourt AMPure XP Reagent

A63881

Agencourt AMPure XP 450 mL Kit 12,500/25,000 preps1 Agencourt AMPure XP Reagent

 

 

 

 

 

 

 

 

 

 

 

 

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